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Přírodovědecká fakulta UPOL

RESEARCH PROJECTS

CURRENTLY RUNNING PROJECTS

Variability of components and interactions in plant pathosystem and impact of environmental factors on their expression

MŠMT ČR, MSM 6998959215 (2005 - 2011)

The research project is focused on complex analysis and understanding of plant pathosystems (or representative models of plant pathosystem) with respect to its structure, hierarchy, variability of its components, interactions, and influence of environment on expression of host-pathogen interactions. There will be emphasize to understand biodiversity and genetic variability of pathogens and host plants, including their temporal and spatial changes. One of the goals of the project is also identification and characterization of new forms of pathogens, and creation of their collection for further investigation, along with detection of resistance sources in the germplasm of cultivated plants and their corresponding wild types. Interaction among plant, pathogen, and environment will be investigated at the level of populations and individuals, on the histological, cytological, and physiological level, and finally, at the biochemical, biophysical, and molecular level. On the level of population and individual, we will look for new insights about spreading and harmfulness of pathogenicity, its temporal and spatial dynamics, about flow of pathogenicity factors, and co-evolutionary relationships between plants and pathogens. At the lower levels, we will endeavor to obtain new information about genetic basis of plant defense mechanisms. Further study will be focused on genetic mechanisms of plant resistance. Molecular and genetic analysis will be aimed on variability of plant pathosystem and its components at the level of polymorphism and mRNA and proteins. Other topic will be identification of the genes or genome segments involved in plant resistance or pathogenesis of phytopathogenic organisms. Finally, investigation will be focused on physiological, biochemical, and biophysical processes affecting, involved and accompanying the interactions between plant, pathogen, and environment. Influence of biotic and abiotic stresses on plant pathosystems will also be analyzed by means of mathematical modeling. Complex insight into structure, variability, and behavior of plant pathogens will be analyzed and interpreted theoretically and practically as well. Also, we assume to propose a strategy of how to apply the information obtained in plant biotechnologies and breeding of plants for resistance to diseases.
Cooperation with Depts Biochemistry, Dept. Cell and Molecular Biology and Dept. Experimental Physics.

Diagnostics and methods of protection against quarantine and other economically important pathogens of fruit and leafy vegetables

MZe ČR (NAZV) QH 71229 (2007 - 2011)

The main aim of the project is contribution to the development of integrate production of fruit and leafy vegetables. The main goal is focused on innovation of plant protection methods (diagnostics, variability of pathogens, efficiency of fungicides and resistance of host plants) against new and quarantine viral, bacterial and fungal plant pathogens. Coordinative workplace: Crop Research Institute, Prague.

Inovation of the metods for sunflower protection

MZe ČR (NAZV) QH 71254 (2007 - 2011)

Project covers several problems related to sunflower production and protection in the Czech Republic. Studies conducted by Dept Botany focus on sunflower downy mildew (Plasmopara halstedii): survey of the disease incidence in the Czech Republic and identification of races of its causal agent, which is inevitable for the effective growing of sunflower resistant hybrids in our country. Coordinative workplace: Crop Research Institute, Prague.

The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture

MZe ČR (1997 - recently)

Within the NP Collection of phytopathogenic microorganisms UPOC is mantained (collection of phytopathogenic fungi, selected phytoplasmas and virus isolates, cyanobacteria and algae of agricultural importance) see https://www.vurv.cz/mikroorganismy/Phytopathogenic%20Microorganisms%20Olomouc.html

Coordination: Crop Research Institute (CRI), Prague-Ruzyně

https://www.vurv.cz/collections/vurv.exe/search

FORMERLY FINISHED PROJECTS

Leafy vegetables germplasm, stimulating use

EU, AGRI GEN RES 2005 (2006 - 2010)

Coordinative workplace: CGN Wageningen (The Netherlands)
Morphological characterisation of leafy vegetables genotypes, evaluation of their reaction to biotic and abiotic factors, organoleptic qualities and possibility of their use in processing industry.

National program for conservation and utilisation of genetic resources of plants and microorganisms with food and agricultural importance
B 3.7 Phytopathogenic microorganisms

MZe ČR, NPGZ-M/03-023 (2004 - 2008)

The program was initiated as a joint platform for conservation and utilisation of genetic resources in Czech republic by Czech Ministry of Agriculture for years 2004-2008.
Coordinative workplace: Výzkumný ústav rostlinné výroby Praha-Ruzyně.

Utilization of protoplast fusion technique in breeding of the important cultivated crops of the genera Brassica, Cucumis and Solanum.

MZe ČR (NAZV) QD 4108 (2004 - 2007)

The main aim of the proposed project is to optimize the unconventional methods of hybridization via protoplast fusion techniques in economically important cultivated crops of the genus Brassica, Cucumis and Solanum. The mothods of protoplasttechnology should help in developing the specific gene combination in studied plant species, which are impossible to obtain through the conventional breeding approaches. Further this techniques offer a convenient way for overcoming the limitations on sexual hybridization in plant breeding. The goal of protoplasts fusion is to control the transfer of an agriculturally important traits, such as disease resistance, abiotic stresses and also the improvement of the quality characteristics of seeds.

The proposed investigations of this research are focussed on the development of the methods for symmetrical and asymmetrical protoplast fusion. The fusions are supposed to be induced through both electroporation (electrical stimulation) as well as chemical fusion of protoplasts in cultivated and wild species of the genus Brassica, Cucumis and Solanum. The studied genotypes will be evaluatedon the basis of the following parameters: protoplast culture efficiency, protoplast viability and regeneration of protoplasts. The step-by- step Process of somatic hybridization will be controlled and analysed employing the cytological methods. The extent of obtained hybrid products from fusions and regenerants will be evaluated by means of cytological as well as molecular biology methods. Developed plant materials should help in broadening the genetical variability in plant species under investigation.

Biology and ecology of the polyploid complex Allium oleraceum L.

GA ČR, GP 206/04/P115 (2004 - 2006)

The objective of the project is a study of selected aspects of the biology and ecology of the polyploid species complex Allium oleraceum L. The following aspects will be analyzed: (a) distribution and habitats demands of the cytotypes of A. oleraceum in selected regions of Europe, (b) estimation of the genome size within this polyploid complex and estimation of the intra- and interpopulation variability in the genome size in relation to geographical and ecological gradients, (c) morphological and anatomical analyses of respective cytotypes, estimation of the growth rate and allocation pattern of the cytotypes and (d) estimation of the phenotypical plasticity in parameters influencing fitness and its importance for ecological and geographical differentiation among cytotypes.

Flora of the Czech Republic - volume 8/2

GA ČR, GA 206/04/0786 (2004 - 2006)

Click on the link above for more informations on web page of the project.

Cytological and biochemical aspects of plant-pathogen interactions (model of Lactuca spp.-Bremia lactucae)

GA ČR, GP 522/02/D011 (2002 - 2005)

Application of new methodological approaches to study the function of plant cell in interaction with pathogen at model study of pathosystem Lactucae spp.-Bremia lactucae.

EU GENE-MINE (QLK5-2000-00722; Quality of life and management of living resources,
Key action 5.1.1. Sustainable agriculture, Sustainable management of resources in agriculture) „Improved use of germplasm collections with the aid of novel methodologies for integration, analysis and presentation of genetic data sets“

EU, GENE-MINE QLK5 -2000 (2001 - 2004)

Nová léčiva závažných lidských onemocnění; dílčí úkol: Stanovení antimikrobiálních účinků chemických látek

MŠMT ČR, MSM 153100008 (1999 - 2004)

Hlavním cílem bylo objasnit zvláštní úlohu purinů, triazinů, ftalocyaninů a strukturálně příbuzných derivátů při růstových regulacích v (ne)nádorových lidských, živočišných a rostlinných buňkách, ale i při replikaci lidských a živočišných virů a bakterií. Konečným cílem byly nové generace protinádorových, antivirálních a antibakteriálních látek s cílenými molekulárními mechanizmy účinku "specificky" indukujícími apoptózu v nádorových buňkách či inhibujícími proliferaci lidských virů či bakterií. Byly vyvíjeny specifické inhibitory cyklin-dependentních kináz, fotosensibilizátory, inhibitory xanthinoxidázy, cytokinioxidázy, DNA virů a klíčových lidských bakterií.

Visual texts for practicals in selected botanical courses

FRVŠ 0038/2004 (2004)

Formation of multimedial learnig texts for practicals in selected botanical subjects (general botany, mycology, phytopathology, vegetation).

Development and utilization of effective methods for selection of genetic resources and production of initial breeding materials of Brassica vegetables with desired traits

MZe ČR (NAZV ) QD 1356 (2001 - 2004)

The aim of the project is to develop efficient methods for selection of genetic resources and production of initial breeding materials of Brassica vegetables with desired agronomic traits parameters. The main criteria for selection of donors will be resistance against important diseases, traits determining quality of products and yield. Promising genotypes of Brassica germplasm collection maintained in Olomouc vegetable genebank will be evaluated for resistance or tolerance to Plasmodiophora brassicae, Peronospora brassicae and Turnip mosaic virus (TuMV) under laboratory as well as under field tests. Recently constructed intergeneric somatic hybrid Armobrassica will be included for parallel screening and properly evaluated for breeder´s use. Methods of artificial infections by specific races of pathogens, growing in infection field and so far not utilized in vitro methods will be used for tests of disease resistance. Immunochemical techiques will be developed and verified for diagnostics of Plasmodiophora brassicae. Agronomic traits will be evaluated in field trials according to standarts for individual crops. Self-incompatibility and CMS will be used for hybrid breeding. To increase efficiency of homozygous doubled haploid lines production the method of microspore culture will be optimised. The methodology of breeding and seed production of line and hybrid varieties of Brassica vegetables using innovated procedures and new initial breeding materials will be worked out.

Flora of the Czech Republic - volume 8

GA ČR, GA 206/01/1115 (2001 - 2003)

  • M. Dančák: treatment of genera Bromopsis, Glyceria a Molinia
  • M. Duchoslav: treatment of genera Allium (in colaboration with F. Krahulec)
  • B. Trávníček: treatment of genera Bromopsis, Glyceria a Scilla
  • R. J. Vašut: co-treatment of genera Taraxacum

Vliv pastvy na biodiverzitu lučních porostů MZCHÚ v CHKO Bílé Karpaty.

MŽP ČR, VaV 610/10/00 (2000 - 2003)

http://old.botany.upol.cz/prezentace/mladek/VaV03.pdf

Biodiversity of the interactions between germplasms of wild Lactuca spp. and related genera and lettuce downy mildew (Bremia lactucae)

OECD (Paris, France) v rámci programu „Biological Resource Management for Sustainable Agricultural Systems“ (1998)

Cytopathological and pathobiochemical aspects of interactions between plants and fungal pathogens
(Model interactions: Lactuca spp.-Bremia lactucae and Pisum sativum-Fusarium spp.)

IG UP (1998)

Virulence distribution, dynamics and diversity in German population of lettuce downy mildew (Bremia lactucae)

Deutsche Forschungsgemeinschaft (Bonn, Germany) (1997)

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